TETRANITRO-BLUE TETRAZOLIUM AND THE ELECTRON HISTOCHEMISTRY OF SUCCINIC DEHYDROGENASE
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چکیده
منابع مشابه
The Use of Tetranitro-blue Tetrazolium for the Cytochemical Localization of Succinic Dehydrogenase
Succinic dehydrogenase (SDH) was localized in the mitochondria of Sarcoma 37 ascites tumor cells by the use of tetranitro-BT (TNBT) and nitro-BT (NBT) in smear preparations. Results with each tetrazolium salt as electron acceptor were evaluated with respect to: (a) size and shape of the formazan precipitate relative to standard mitochondrial morphology; (b) crystallization phenomena of reduced ...
متن کاملThe Demonstration of the Succinic Dehydrogenase System in Bacillus Subtilis Using Tetranitro-blue Tetrazolium Combined with Techniques of Electron Microscopy
Activity of the succinic dehydrogenase system was studied in Bacillus subtilis utilizing combined techniques of cytochemistry and electron microscopy. Organisms were incubated in a medium containing tetranitro-blue tetrazolium (TNBT) which served as an electron acceptor. Enzymatic activity, as evidenced by deposition of TNBT-formazan, was found on membranous organelles associated with the cytop...
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In Bacillus subtilis the reduction of potassium tellurite and of tetranitro-blue tetrazolium (TNBT) give comparable results: reduced products appear deposited in particular membranous organelles. For this reason these specialized organelles are considered to be chondrioids (1-3). In the Gram-negative Proteus vulgaris similar membranous structures were never observed by us (4, 5). Therefore it i...
متن کاملTetranitro-blue Tetrazolium Reduction in Bacillus Subtilis
In order to decide whether in the bacterial cell the enzymes of the respiratory chain are located in "particulate elements" (1) or in the plasma membrane (2) we investigated the reduction of potassium tellurite in Gram-positive and Gram-negative bacteria. For the first time in 1962 (3), and in more detail in 1964 (4), we reported that with Bacillus subtilis we had found that reduction products ...
متن کاملLocalization of the Succinic Dehydrogenase System in Escherichia Coli Using Combined Techniques of Cytochemistry and Electron Microscopy
The activity of the succinic dehydrogenase system was studied in Escherichia coli utilizing combined techniques of cytochemistry and electron microscopy. Organisms were incubated in a medium containing tetranitro-blue tetrazolium (TNBT) which served as an electron acceptor. Enzymatic activity, as evidenced by the deposition of aggregates of TNBT-formazan, was found associated with the site of t...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1962
ISSN: 0022-1554,1551-5044
DOI: 10.1177/10.4.506